Aleix Espargaro najszybszym zawodnikiem podczas drugiej sesji treningowej do Grand Prix Hiszpanii na torze w Jerez. Drugi rezultat uzyskał kolega zespołowy Hiszpana, Maverick Vinales. trzeci rezultat uzyskał Jack Miller.
| POS | RIDER | NAT | TEAM | TIME/DIFF | LAP | MAX | |
| 1 | ^4 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP23) | 1’36.708s | 21/22 | 290k |
| 2 | ^8 | Maverick Viñales | SPA | Aprilia Racing (RS-GP23) | +0.002s | 24/25 | 293k |
| 3 | ^5 | Jack Miller | AUS | Red Bull KTM (RC16) | +0.127s | 26/27 | 293k |
| 4 | ^7 | Johann Zarco | FRA | Pramac Ducati (GP22) | +0.188s | 21/22 | 290k |
| 5 | ^8 | Miguel Oliveira | POR | RNF Aprilia (RS-GP22) | +0.248s | 24/25 | 291k |
| 6 | ^1 | Alex Marquez | SPA | Gresini Ducati (GP22) | +0.333s | 22/23 | 290k |
| 7 | ^7 | Marco Bezzecchi | ITA | Mooney VR46 Ducati (GP22) | +0.466s | 23/24 | 293k |
| 8 | ^1 | Francesco Bagnaia | ITA | Ducati Lenovo (GP23) | +0.525s | 20/21 | 290k |
| 9 | ˅8 | Dani Pedrosa | SPA | Red Bull KTM (RC16) | +0.543s | 23/24 | 298k |
| 10 | ˅6 | Luca Marini | ITA | Mooney VR46 Ducati (GP22) | +0.568s | 22/25 | 290k |
| 11 | ˅9 | Jorge Martin | SPA | Pramac Ducati (GP23) | +0.598s | 24/28 | 293k |
| 12 | ˅6 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.682s | 21/24 | 293k |
| 13 | ^4 | Raul Fernandez | SPA | RNF Aprilia (RS-GP22) | +0.695s | 19/20 | 289k |
| 14 | ˅11 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +0.775s | 25/26 | 288k |
| 15 | ^3 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.797s | 25/28 | 292k |
| 16 | ^4 | Franco Morbidelli | ITA | Monster Yamaha (YZR-M1) | +0.992s | 21/25 | 291k |
| 17 | ^2 | Fabio Di Giannantonio | ITA | Gresini Ducati (GP22) | +1.152s | 19/24 | 288k |
| 18 | ˅2 | Stefan Bradl | GER | Team HRC (RC213V) | +1.165s | 24/25 | 290k |
| 19 | ˅4 | Joan Mir | SPA | Repsol Honda (RC213V) | +1.208s | 22/22 | 288k |
| 20 | ^2 | Enea Bastianini | ITA | Ducati Lenovo (GP23) | +1.277s | 17/17 | 291k |
| 21 | ˅9 | Alex Rins | SPA | LCR Honda (RC213V) | +1.424s | 22/24 | 289k |
| 22 | ^1 | Jonas Folger | GER | Tech3 GASGAS (RC16) | +1.990s | 19/20 | 289k |
| 23 | ˅2 | Augusto Fernandez | SPA | Tech3 GASGAS (RC16)* | +2.220s | 21/24 | 287k |
| 24 | = | Iker Lecuona | SPA | Repsol Honda (RC213V) | +2.566s | 21/24 | 288k |