Francesco Bagnaia z czasem 1:30.031 ustanowił najszybszy czas jednego okrążenia podczas kwalifikacji do Grand Prix Emilia-Romagna na torze w Misano. Aktualny mistrz Świata ustanowił również rekord jednego okrążenia na tym torze. Pierwszy rząd uzupełni Jorge Martin i Enea Bastianini! Marc Marquez dopiero z siódmym rezultatem i z wywrotką w Q2.
| Pos | Rider | Nat | Team | Time/Diff | Lap | Max |
| 1 | Francesco Bagnaia | ITA | Ducati Lenovo (GP24) | 1’30.031s | 7/8 | 302k |
| 2 | Jorge Martin | SPA | Pramac Ducati (GP24) | +0.214s | 3/8 | 304k |
| 3 | Enea Bastianini | ITA | Ducati Lenovo (GP24) | +0.533s | 8/8 | 299k |
| 4 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.605s | 7/8 | 303k |
| 5 | Pedro Acosta | SPA | Red Bull GASGAS Tech3 (RC16)* | +0.700s | 3/8 | 303k |
| 6 | Marco Bezzecchi | ITA | VR46 Ducati (GP23) | +0.806s | 7/8 | 305k |
| 7 | Marc Marquez | SPA | Gresini Ducati (GP23) | +0.849s | 3/7 | 298k |
| 8 | Maverick Viñales | SPA | Aprilia Racing (RS-GP24) | +0.878s | 6/8 | 298k |
| 9 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.890s | 9/9 | 295k |
| 10 | Franco Morbidelli | ITA | Pramac Ducati (GP24) | +0.901s | 6/7 | 302k |
| 11 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP24) | +1.006s | 7/8 | 301k |
| 12 | Miguel Oliveira | POR | Trackhouse Aprilia (RS-GP24) | +1.083s | 7/8 | 304k |
| Qualifying 1: | ||||||
| 13 | Fabio Di Giannantonio | ITA | VR46 Ducati (GP23) | 1’31.285s | 7/8 | 298k |
| 14 | Raul Fernandez | SPA | Trackhouse Aprilia (RS-GP24) | 1’31.402s | 6/8 | 298k |
| 15 | Luca Marini | ITA | Repsol Honda (RC213V) | 1’31.428s | 7/8 | 298k |
| 16 | Joan Mir | SPA | Repsol Honda (RC213V) | 1’31.45s | 7/8 | 302k |
| 17 | Johann Zarco | FRA | LCR Honda (RC213V) | 1’31.501s | 7/8 | 300k |
| 18 | Augusto Fernandez | SPA | Red Bull GASGAS Tech3 (RC16) | 1’31.554s | 6/6 | 298k |
| 19 | Jack Miller | AUS | Red Bull KTM (RC16) | 1’31.695s | 7/8 | 300k |
| 20 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | 1’32.061s | 8/9 | 296k |
| 21 | Alex Marquez | SPA | Gresini Ducati (GP23) | 1’32.332s | 2/7 | 299k |
| Alex Rins | SPA | Monster Yamaha (YZR-M1) | No Time | 0/0 | – |
