Jack Miller najszybszym zawodnikiem podczas pierwszej sesji treningowej do Grand Prix Japonii na torze Motegi. Drugi czas ustanowił Francesco Bagnaia, trzeci był Fabio Quartararo.
| POS | RIDER | NAT | TEAM | TIME/DIFF | LAP | MAX |
| 1 | Jack Miller | AUS | Ducati Lenovo (GP22) | 1’44.509s | 25/28 | 311k |
| 2 | Francesco Bagnaia | ITA | Ducati Lenovo (GP22) | +0.028s | 24/26 | 313k |
| 3 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.049s | 28/30 | 310k |
| 4 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP) | +0.068s | 25/26 | 310k |
| 5 | Luca Marini | ITA | Mooney VR46 Ducati (GP22) | +0.136s | 26/27 | 312k |
| 6 | Marc Marquez | SPA | Repsol Honda (RC213V) | +0.147s | 23/26 | 311k |
| 7 | Pol Espargaro | SPA | Repsol Honda (RC213V) | +0.169s | 24/26 | 312k |
| 8 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.226s | 29/30 | 311k |
| 9 | Miguel Oliveira | POR | Red Bull KTM (RC16) | +0.234s | 27/28 | 310k |
| 10 | Maverick Viñales | SPA | Aprilia Racing (RS-GP) | +0.288s | 24/25 | 311k |
| 11 | Johann Zarco | FRA | Pramac Ducati (GP22) | +0.289s | 24/25 | 314k |
| 12 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +0.377s | 26/28 | 307k |
| 13 | Alex Rins | SPA | Suzuki Ecstar (GSX-RR) | +0.404s | 27/31 | 309k |
| 14 | Enea Bastianini | ITA | Gresini Ducati (GP21) | +0.469s | 19/24 | 312k |
| 15 | Jorge Martin | SPA | Pramac Ducati (GP22) | +0.500s | 26/28 | 313k |
| 16 | Franco Morbidelli | ITA | Monster Yamaha (YZR-M1) | +0.556s | 24/28 | 306k |
| 17 | Cal Crutchlow | GBR | WithU Yamaha RNF (YZR-M1) | +0.678s | 23/27 | 308k |
| 18 | Darryn Binder | RSA | WithU Yamaha RNF (YZR-M1)* | +0.857s | 27/30 | 307k |
| 19 | Marco Bezzecchi | ITA | Mooney VR46 Ducati (GP21)* | +0.917s | 27/29 | 312k |
| 20 | Alex Marquez | SPA | LCR Honda (RC213V) | +1.091s | 29/30 | 309k |
| 21 | Raul Fernandez | SPA | KTM Tech3 (RC16)* | +1.230s | 25/27 | 309k |
| 22 | Tetsuta Nagashima | JPN | HRC Team (RC213V) | +1.336s | 22/24 | 304k |
| 23 | Remy Gardner | AUS | KTM Tech3 (RC16)* | +1.482s | 25/27 | 308k |
| 24 | Fabio Di Giannantonio | ITA | Gresini Ducati (GP21)* | +1.856s | 17/24 | 311k |
| 25 | Takuya Tsuda | JPN | Suzuki Ecstar (GSX-RR) | +2.209s | 22/22 | 304k |