Jorge Martin najszybszy w drugiej sesji treningowej na torze w Austin. Drugi rezultat uzyskał Francesco Bagnaia, trzeci był Alex Rins.
Do Q2 nie zakwalifikował się lider klasyfikacji generalnej, Marco Bezzecchi, Joan Mir, Takaaki Nakagami, Johann Zarco, Miguel Oliveira, Franco Morbidelli, Michele Pirro, Raul Fernandez, Fabio Di Giannantonio, Stefan Bradl, Augusto Fernandez i Jonas Folger.
| POS | RIDER | NAT | TEAM | TIME/DIFF | LAP | MAX | |
| 1 | ^5 | Jorge Martin | SPA | Pramac Ducati (GP23) | 2’02.178s | 19/20 | 341k |
| 2 | ^5 | Francesco Bagnaia | ITA | Ducati Lenovo (GP23) | +0.063s | 20/21 | 341k |
| 3 | ^6 | Alex Rins | SPA | LCR Honda (RC213V) | +0.217s | 19/20 | 342k |
| 4 | ˅3 | Luca Marini | ITA | Mooney VR46 Ducati (GP22) | +0.238s | 20/21 | 343k |
| 5 | ˅1 | Alex Marquez | SPA | Gresini Ducati (GP22) | +0.504s | 20/21 | 340k |
| 6 | ^2 | Maverick Viñales | SPA | Aprilia Racing (RS-GP23) | +0.539s | 22/22 | 342k |
| 7 | ˅5 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.543s | 19/20 | 338k |
| 8 | ^3 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.557s | 18/18 | 344k |
| 9 | ^9 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP23) | +0.587s | 19/20 | 342k |
| 10 | ˅5 | Jack Miller | AUS | Red Bull KTM (RC16) | +0.663s | 18/19 | 340k |
| 11 | ^3 | Marco Bezzecchi | ITA | Mooney VR46 Ducati (GP22) | +0.700s | 20/22 | 343k |
| 12 | = | Joan Mir | SPA | Repsol Honda (RC213V) | +0.726s | 16/17 | 339k |
| 13 | ˅3 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +1.030s | 20/20 | 335k |
| 14 | ˅11 | Johann Zarco | FRA | Pramac Ducati (GP22) | +1.151s | 17/17 | 339k |
| 15 | = | Miguel Oliveira | POR | RNF Aprilia (RS-GP22) | +1.162s | 14/14 | 340k |
| 16 | ˅3 | Franco Morbidelli | ITA | Monster Yamaha (YZR-M1) | +1.265s | 14/18 | 339k |
| 17 | ^4 | Michele Pirro | ITA | Ducati Lenovo (GP23) | +1.507s | 17/18 | 341k |
| 18 | ˅2 | Raul Fernandez | SPA | RNF Aprilia (RS-GP22) | +1.512s | 17/19 | 339k |
| 19 | ˅2 | Fabio Di Giannantonio | ITA | Gresini Ducati (GP22) | +1.901s | 16/17 | 340k |
| 20 | = | Stefan Bradl | GER | Repsol Honda (RC213V) | +1.951s | 14/18 | 333k |
| 21 | ˅2 | Augusto Fernandez | SPA | Tech3 GASGAS (RC16)* | +2.383s | 15/16 | 338k |
| 22 | = | Jonas Folger | GER | Tech3 GASGAS (RC16) | +4.351s | 17/17 | 334k |