Luca Marini najszybszy w drugiej sesji treningowej do Grand Prix Walencji! Drugi rezultat ustanowił Jorge Martin, trzeci Jack Miller. Fabio Quartararo z ósmym czasem, Francesco Bagnaia tuż za nim ze stratą 0,005 sekundy!
| POS | RIDER | NAT | TEAM | TIME/DIFF | LAP | MAX | |
| 1 | ^10 | Luca Marini | ITA | Mooney VR46 Ducati (GP22) | 1’30.217s | 19/21 | 326k |
| 2 | ^5 | Jorge Martin | SPA | Pramac Ducati (GP22) | +0.105s | 19/20 | 329k |
| 3 | ^1 | Jack Miller | AUS | Ducati Lenovo (GP22) | +0.128s | 17/18 | 331k |
| 4 | ˅2 | Marc Marquez | SPA | Repsol Honda (RC213V) | +0.173s | 18/19 | 327k |
| 5 | ^9 | Enea Bastianini | ITA | Gresini Ducati (GP21) | +0.177s | 19/20 | 331k |
| 6 | ^2 | Miguel Oliveira | POR | Red Bull KTM (RC16) | +0.205s | 20/21 | 326k |
| 7 | ˅1 | Johann Zarco | FRA | Pramac Ducati (GP22) | +0.207s | 21/21 | 324k |
| 8 | ˅7 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | +0.225s | 20/21 | 326k |
| 9 | ^8 | Francesco Bagnaia | ITA | Ducati Lenovo (GP22) | +0.230s | 19/20 | 327k |
| 10 | ˅7 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.302s | 20/20 | 329k |
| 11 | ˅6 | Alex Rins | SPA | Suzuki Ecstar (GSX-RR) | +0.490s | 21/22 | 327k |
| 12 | ^4 | Maverick Viñales | SPA | Aprilia Racing (RS-GP) | +0.597s | 18/19 | 327k |
| 13 | ˅4 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP) | +0.673s | 17/17 | 327k |
| 14 | ˅4 | Joan Mir | SPA | Suzuki Ecstar (GSX-RR) | +0.787s | 17/18 | 326k |
| 15 | ^5 | Marco Bezzecchi | ITA | Mooney VR46 Ducati (GP21)* | +0.797s | 20/21 | 327k |
| 16 | ˅3 | Franco Morbidelli | ITA | Monster Yamaha (YZR-M1) | +0.799s | 14/15 | 323k |
| 17 | ^1 | Alex Marquez | SPA | LCR Honda (RC213V) | +0.931s | 18/19 | 326k |
| 18 | ^1 | Pol Espargaro | SPA | Repsol Honda (RC213V) | +1.032s | 17/19 | 326k |
| 19 | ^2 | Cal Crutchlow | GBR | WithU Yamaha RNF (YZR-M1) | +1.128s | 19/19 | 324k |
| 20 | ˅8 | Raul Fernandez | SPA | KTM Tech3 (RC16)* | +1.381s | 18/19 | 323k |
| 21 | ˅6 | Remy Gardner | AUS | KTM Tech3 (RC16)* | +1.404s | 19/20 | 323k |
| 22 | ^1 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +1.455s | 20/21 | 323k |
| 23 | ˅1 | Fabio Di Giannantonio | ITA | Gresini Ducati (GP21)* | +1.545s | 12/13 | 326k |
| 24 | = | Darryn Binder | RSA | WithU Yamaha RNF (YZR-M1)* | +2.203s | 20/20 | 327k |