Francesco Bagnaia z rekordem toru i pole position do Grand Prix Holandii na torze TT Assen! Pierwszy rząd uzupełni Jorge Martin i Maverick Vinales.
| Pos | Rider | Nat | Team | Time/Diff | Lap | Max |
| 1 | Francesco Bagnaia | ITA | Ducati Lenovo (GP24) | 1’30.540s | 5/7 | 310k |
| 2 | Jorge Martin | SPA | Pramac Ducati (GP24) | +0.081s | 8/8 | 312k |
| 3 | Maverick Viñales | SPA | Aprilia Racing (RS-GP24) | +0.411s | 6/7 | 311k |
| 4 | Alex Marquez | SPA | Gresini Ducati (GP23) | +0.439s | 8/8 | 311k |
| 5 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP24) | +0.537s | 6/6 | 311k |
| 6 | Fabio Di Giannantonio | ITA | VR46 Ducati (GP23) | +0.734s | 2/5 | 308k |
| 7 | Marc Marquez | SPA | Gresini Ducati (GP23) | +0.838s | 2/6 | 312k |
| 8 | Franco Morbidelli | ITA | Pramac Ducati (GP24) | +0.865s | 7/8 | 311k |
| 9 | Brad Binder | RSA | Red Bull KTM (RC16) | +0.939s | 8/8 | 313k |
| 10 | Pedro Acosta | SPA | Red Bull GASGAS Tech3 (RC16)* | +0.942s | 7/7 | 314k |
| 11 | Enea Bastianini | ITA | Ducati Lenovo (GP24) | +1.088s | 3/8 | 312k |
| 12 | Raul Fernandez | SPA | Trackhouse Aprilia (RS-GP23) | +1.388s | 6/8 | 308k |
| Qualifying 1: | ||||||
| 13 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | 1’31.62s | 7/8 | 304k |
| 14 | Jack Miller | AUS | Red Bull KTM (RC16) | 1’31.903s | 7/8 | 311k |
| 15 | Marco Bezzecchi | ITA | VR46 Ducati (GP23) | 1’31.997s | 3/6 | 311k |
| 16 | Alex Rins | SPA | Monster Yamaha (YZR-M1) | 1’32.108s | 7/9 | 304k |
| 17 | Miguel Oliveira | POR | Trackhouse Aprilia (RS-GP24) | 1’32.123s | 7/9 | 315k |
| 18 | Lorenzo Savadori | ITA | Aprilia Racing (RS-GP24) | 1’32.243s | 7/8 | 308k |
| 19 | Johann Zarco | FRA | LCR Honda (RC213V) | 1’32.26s | 7/8 | 306k |
| 20 | Joan Mir | SPA | Repsol Honda (RC213V) | 1’32.497s | 7/9 | 307k |
| 21 | Luca Marini | ITA | Repsol Honda (RC213V) | 1’32.627s | 7/9 | 309k |
| 22 | Augusto Fernandez | SPA | Red Bull GASGAS Tech3 (RC16) | 1’32.669s | 7/9 | 308k |
| 23 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | 1’33.03s | 3/9 | 303k |
