Fabio Quartararo ustanawia najszybszy czas okrążenia podczas drugiego treningu wolnego w Silverstone. Lider klasyfikacji generalnej jako jedyny zszedł poniżej 1:59. Czołową piątkę zamykają Mir, Vinales, Zarco oraz Aleix Espargaro.
| POS | RIDER | NAT | TEAM | TIME/DIFF | LAP | MAX | |
| 1 | ^3 | Fabio Quartararo | FRA | Monster Yamaha (YZR-M1) | 1’58.946s | 14/15 | 331k |
| 2 | ^6 | Joan Mir | SPA | Suzuki Ecstar (GSX-RR) | +0.154s | 15/16 | 333k |
| 3 | ^4 | Maverick Viñales | SPA | Aprilia Racing (RS-GP) | +0.177s | 15/15 | 329k |
| 4 | ˅3 | Johann Zarco | FRA | Pramac Ducati (GP22) | +0.188s | 15/15 | 327k |
| 5 | ^1 | Aleix Espargaro | SPA | Aprilia Racing (RS-GP) | +0.207s | 14/15 | 329k |
| 6 | ^8 | Miguel Oliveira | POR | Red Bull KTM (RC16) | +0.217s | 15/15 | 330k |
| 7 | ˅4 | Alex Rins | SPA | Suzuki Ecstar (GSX-RR) | +0.300s | 11/18 | 330k |
| 8 | ^8 | Enea Bastianini | ITA | Gresini Ducati (GP21) | +0.412s | 15/16 | 329k |
| 9 | ^3 | Jack Miller | AUS | Ducati Lenovo (GP22) | +0.418s | 13/14 | 332k |
| 10 | ^3 | Marco Bezzecchi | ITA | Mooney VR46 Ducati (GP21)* | +0.432s | 17/17 | 332k |
| 11 | ˅9 | Francesco Bagnaia | ITA | Ducati Lenovo (GP22) | +0.439s | 14/15 | 331k |
| 12 | ^7 | Luca Marini | ITA | Mooney VR46 Ducati (GP22) | +0.450s | 15/16 | 334k |
| 13 | ˅4 | Jorge Martin | SPA | Pramac Ducati (GP22) | +0.711s | 15/16 | 331k |
| 14 | ^1 | Alex Marquez | SPA | LCR Honda (RC213V) | +0.843s | 14/16 | 327k |
| 15 | ^7 | Stefan Bradl | GER | Repsol Honda (RC213V) | +0.855s | 16/16 | 328k |
| 16 | ˅5 | Pol Espargaro | SPA | Repsol Honda (RC213V) | +0.906s | 13/14 | 332k |
| 17 | ^6 | Fabio Di Giannantonio | ITA | Gresini Ducati (GP21)* | +1.040s | 15/16 | 327k |
| 18 | ˅13 | Takaaki Nakagami | JPN | LCR Honda (RC213V) | +1.057s | 4/16 | 326k |
| 19 | ^1 | Andrea Dovizioso | ITA | WithU Yamaha RNF (YZR-M1) | +1.097s | 15/16 | 328k |
| 20 | ^1 | Franco Morbidelli | ITA | Monster Yamaha (YZR-M1) | +1.192s | 15/17 | 326k |
| 21 | ˅11 | Brad Binder | RSA | Red Bull KTM (RC16) | +1.198s | 4/10 | 324k |
| 22 | ˅5 | Remy Gardner | AUS | KTM Tech3 (RC16)* | +1.208s | 14/15 | 330k |
| 23 | ^1 | Darryn Binder | RSA | WithU Yamaha RNF (YZR-M1)* | +2.582s | 2/10 | 326k |
| 24 | ˅6 | Raul Fernandez | SPA | KTM Tech3 (RC16)* | +3.116s | 7/11 | 329k |